Fig. 2.
Characterization of lipid monolayer topography and giant unilamellar vesicles (GUV) composed of dioleoylphosphatidylcholine (DOPC):sphingomyelin (SM):sterols (1:1:1), with the sterols being of different chemical nature. a, e DOPC:egg yolk (e) SM:cholesterol (Chol), b, f DOPC:eSM:ergosterol (Erg), c, g DOPC:eSM:Cln, d, h DOPC:eSM. a–d Visualization of lipid monolayers by Brewster angle microscopy. Images were taken at 20 mN m−1. For better visualization, the lower 0–90 Gy level range (from the 0 to 255 original scale) was selected to maintain the gray level:film thickness ratio. Scale bar (d) 100 μm, also for images a–c. Insets to a and b show a piece of both images enhanced by a bandpass filter. e–h Visualization of GUV by confocal microscopy. All vesicles contain the fluorescence probe DiD (0.1 mol%). Scale bars (e–h) 5 μm. T = 23 ± 2 °C