FIG. 2.

Cell proliferation activity was limited to the first 48 h of adipogenic induction. (A) Cells were plated at equal density across different treatment groups (triplicate wells per treatment group) before media treatment. Proliferating cells were labeled by EdU, a thymidine analog, which was added 8 h before the designated fixation hours, and detected by Alexa Fluor 488 tagged Azide-containing detection reagent (green). All cells were stained with DAPI (blue). (B) Percentage of proliferating cells was determined at 12, 24, 48, 72, 96, 120, 144, and 168 h after treatment initiation in CM, DEX, AIM, or OIM (n = 3). EdU, 5-ethynyl-2′-deoxyuridine; OIM, osteogenic inducing media. Color images available online at www.liebertpub.com/scd