FIG. 5.

SiCdk1 significantly enhanced adipogenic differentiation of hMSCs, while inhibiting its proliferation. (A) Equal number of cells was transfected with siCon, siCDK1, or siCDK2 at 20 h before AIM treatment initiation, followed by AIM change at 48-h intervals. OilRedO quantification and DAPI cell counts were determined after 12 days of AIM treatment and values for each treatment group were graphed after they were normalized against those of the siCon group. (B) After 12 days of AIM treatment, whole well images of siCon, siCdk1, or siCdk2 stained with OilRedO solution were taken. (C) Cell proliferation rate in siCon and siCdk1 transfected cells was determined by EdU labeling at the designated time points after AIM treatment initiation. *P < 0.01 (n = 3). hMSCs, human mesenchymal stem cells; siCon, SiControl. Color images available online at www.liebertpub.com/scd