FIG. 6.

The effect of siCCND1 and siCdk4 on adipogenic differentiation of hMSCs was weaker than those of siCdk1. (A) Equal number of cells was transfected with siCon, siCdk, or siCCND1 separately, or siCon, siCdk1, or siCdk4 separately, at 20 h before AIM treatment initiation, followed by AIM change at 48-h intervals. OilRedO quantification, adipocyte counts, and DAPI cell counts were determined after 12 days of AIM treatment, and values for each treatment group were graphed after they were normalized against those of the siCon group. (B) Representative whole well images of different treatment groups stained with OilRedO solution were taken after 12 days of AIM treatment. (C) Images merging both OilRedO and DAPI stain allowed better visual distinction of individual adipocytes. Images were taken from the same positions within siCon- and siCdk-transfected wells, depicting a significantly greater percentage of adipocytes forming in siCdk wells (24/40) compared to those in siCon wells (14/49). The average differentiation efficiency of the former is about 1.5-fold over the latter. *P < 0.01; **P ≤ 0.05; ***P ≤ 0.1 (n = 3). Color images available online at www.liebertpub.com/scd