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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Protein Expr Purif. 2016 Jul 5;127:53–60. doi: 10.1016/j.pep.2016.07.002

Figure 2. Detergent screening for KcsA solubilization.

Figure 2

A Western blot analysis shows tetrameric KcsA running as a 50 kDa molecular weight complex. A. The different lanes are showing KcsA extracted from E. coli membranes using various detergents. B. Western blot showing the extraction of KcsA using Triton X-100 while increasing either NaCl or KCl concentration. C. Quantification by densitometric analysis of the effect of increasing salt concentration on KcsA solubilization per detergent type (Fig. 2A). D. Densitometric analysis to quantify the effect on KcsA extraction from E. coli membrane by Triton X-100 while increasing the NaCl or KCl concentration (Fig 2B).