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. 2017 Oct 30;12(10):e0186985. doi: 10.1371/journal.pone.0186985

Table 1. Primers and probes used in this study.

PCR types Primer/Probe
names
Sequence (5’-3’) TM (°C)
Conventional PCR F2 Fam-gacacataggtgaggtct 56
R2 aacggctgatgaccaaact 56
P aggaccacgatcgctgat-Biotin 59
One-tube nested PCR F1 ctgcgagcgtaggcgtcggtgacaaa 72
R1 ctgaaccgtgagggcatcgaggtggc 72
F2 Fam-gacacataggtgaggtct 56
R2 aacggctgatgaccaaact 56
P aggaccacgatcgctgat-Biotin 59