Table 1. Primers and probes used in this study.
PCR types | Primer/Probe names |
Sequence (5’-3’) | TM (°C) |
---|---|---|---|
Conventional PCR | F2 | Fam-gacacataggtgaggtct | 56 |
R2 | aacggctgatgaccaaact | 56 | |
P | aggaccacgatcgctgat-Biotin | 59 | |
One-tube nested PCR | F1 | ctgcgagcgtaggcgtcggtgacaaa | 72 |
R1 | ctgaaccgtgagggcatcgaggtggc | 72 | |
F2 | Fam-gacacataggtgaggtct | 56 | |
R2 | aacggctgatgaccaaact | 56 | |
P | aggaccacgatcgctgat-Biotin | 59 |