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. Author manuscript; available in PMC: 2018 Aug 18.
Published in final edited form as: ACS Chem Biol. 2017 Jul 14;12(8):1999–2007. doi: 10.1021/acschembio.7b00242

Figure 1. Effects with SSOs.

Figure 1

(a) Structure of UNC2383. (b) UNC2383 enhances SSO effects. HeLa Luc 705 cells were incubated in 24-well plates with 100 nM SSO623 or its mismatched control (MM) for 16 h in DMEM +10% FBS, rinsed and then treated with various concentrations of UNC2383 for 2 h. The cells were then rinsed and incubation continued for an additional 4 h in DMEM +10% FBS. Cells were rinsed twice in PBS and luciferase activity (RLU) and cell protein determined. The larger round symbol indicates the MM oligonucleotide. Means +/− SE. N = 3. Cell protein data for this assay is in Supporting Table 2. (c) Kinetics of UNC2383 action. Cells were preloaded with 100 nM SSO623 and then exposed to UNC2383 for various periods after which the compound was removed. The cells were further incubated and then luciferase and protein determined. The total time of incubation in each case was 6 h. (d) Cytotoxicity of UNC2383. HeLa Luc705 or NIH-3T3-MDR cells were exposed to UNC2383 as in (b) then incubated for 24 h in DMEM plus 10% FBS and tested using the Alamar Blue cytotoxicity assay. Means +/− SE. N = 3.