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. Author manuscript; available in PMC: 2017 Oct 30.
Published in final edited form as: Lab Invest. 2013 Jan 28;93(3):365–374. doi: 10.1038/labinvest.2012.178

FIGURE 4. Monoclonal antibodies from clones K117 and 5E10 recognize epitopes on Thy-1 independent of its glycosylation, but are abolished under reducing conditions.

FIGURE 4

Mature Thy-1 detected by western blot in equal dilutions of untreated or PNGase F treated NHLF extract prepared as indicated under NR conditions or with 100mM DTT (DTT) or 2.5% β-mercaptoethanol (β).