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. Author manuscript; available in PMC: 2017 Oct 30.
Published in final edited form as: Lab Invest. 2013 Jan 28;93(3):365–374. doi: 10.1038/labinvest.2012.178

FIGURE 5. Characterization of soluble recombinant Thy-1 with 6 histidine epitope tag at C-terminus.

FIGURE 5

(A) Mature Thy-1 detected by western blot in the CE of NHLF or CM containing sTHY1 – 6X HIS prepared as indicated under NR conditions or with 2.5% β-mercaptoethanol (β). After stripping the membranes of antibodies for detecting mature Thy-1, the 6X HIS epitope tag was probed for. (B) 6X HIS epitope detected by western blot in equal dilutions of untreated or PNGase F treated CM containing sThy-1 – 6X HIS.