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. Author manuscript; available in PMC: 2017 Oct 30.
Published in final edited form as: Lab Invest. 2013 Jan 28;93(3):365–374. doi: 10.1038/labinvest.2012.178

FIGURE 6. Thy-1 in the Conditioned Media of Normal Human Lung Fibroblasts is insoluble.

FIGURE 6

(A) CM was collected from CCL-210 provided either MEM or 20ng/mL TNFα and IL-1β in MEM for 24 to 48 hrs. Using Triton X-114, concentrated CM was partitioned into an aqueous (AP) and insoluble phase (IP). Mature Thy-1 detected by western blot in the CE of NHLF or partitioned and pre-partitioned CM by western blot using K117. (B) CM was collected from CCL-210 provided MEM 48 hrs. CM was submitted to differential centrifugation of 1,200 × g and 21,000 × g then precipitated with methanol. Mature Thy-1 was detected in the precipitated material of total (T) prior to, supernatant (S) after, and pellet (P) after 21,000 × g by western blot using K117.