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. 2017 Oct 30;12(10):e0187051. doi: 10.1371/journal.pone.0187051

Fig 5. SEC-MALS analysis of cT1R1-NTD.

Fig 5

The column was equilibrated and cT1R1-NTD eluted with 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.1 mM DDM. The chromatograms show the readings of the light scattering (LS), the differential refractive index (dRI) and UV detectors in red, blue and green, respectively. The scale for the LS detector is shown in the right-hand axis. The thick black line indicates the calculated molecular mass of the eluting protein throughout the chromatogram (scale on the left-hand axis). cT1R1-NTD has a fitted molecular mass of 52.5 kDa; its theoretical monomer molecular mass value is 55.4 kDa.