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. 2017 Oct 30;12(10):e0187051. doi: 10.1371/journal.pone.0187051

Fig 6. cT1R1-NTD binds L-amino acids and IMP.

Fig 6

Normalized maximal fluorescence intensity of cT1R1-NTD before and after addition of ligands (100 μM final concentration). L-Cys does not affect cT1R1-NTD fluorescence. Fluorescence of cT1R1-NTD alone was defined as 100% in absence of ligand. Excitation and emission wavelength were 295 nm and 340 nm, respectively. cT1R1-NTD concentration was 0.5 μM. Data values are the mean ± SEMs of more than nine independent replicates of at least three independently refolded protein samples. *, Significantly different from cT1R1-NTD before addition of ligands (one-way ANOVA followed by Dunnett’s, p ≤ 0.05; for L-Arg p ≤ 0.08).