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. 2017 Oct 18;11(10):e0005981. doi: 10.1371/journal.pntd.0005981

Fig 2. Validation of the miRNA expression profile.

Fig 2

(A) miR-3960 and miR-4508 expression was determined by real-time PCR using specific primers for the mature form of the miRNAs. Fold changes of the respective comparisons were determined through the comparative Ct method taking into account the PCR efficiency and miRNA-16-5p as reference miRNA. Data is presented as mean ± SEM from three different blood donors. (B) Spearman correlation between the fold changes obtained by RNA sequencing and real-time PCR.