Fig 3. Effect of miR-181a, miR-4301, miR-3960 and miR-4508 on DENV infection of Huh7 cells.

Huh7 cells were transfected with a mimic negative control (NC) and with the indicated miRNA mimics. At 24 hpt, cells were infected at MOIs 1, 5 and 10. The percentage of E-positive cells (grey bars) and infectious virus particle production (black bars) were determined at 24 hpi. The percentages of infection of cells transfected with the NC and infected at MOIs 1, 5 and 10 were 6.57±2.07; 13.9±3.48 and 18.53±2.64 respectively; while the viral titers were 1.2x10⁵±5x10⁴; 1.08x10⁶±2x10⁵ and 7.06 x10⁶±6x10⁴ PFU/ml respectively. Data is presented as the percentage relative to the NC and shows mean ± SEM from three independent experiments. Statistical differences were assessed with Student’s t-test.