Fig 4. Effect of miR-3614-5p on DENV and WNV infection of Huh7 cells.

Huh7 cells were transfected as described in the legend to Fig 3. (A) Infectious virus particle production at 24 hpi. In cells transfected with NC and infected at MOI 1, 5 and 10 the viral titers were 1.2x10⁵±5x10⁴; 1.08x10⁶±2x10⁵ and 7.06x10⁶±6x10⁴ PFU/ml respectively. (B) Number of released DENV genome equivalent particles at 24 hpi. For NC-transfected cells infected at MOIs 1, 5 and 10 the DENV genome equivalent particles per ml were: 2.33x10⁷±1x10⁷; 6.77x10⁷±3x10⁷ and 1.72x10⁸±5x10⁷ respectively. (C) Infectious DENV production following infection at MOI 5 and harvesting times of 24, 36, 48 hpi. The corresponding viral titers of cells transfected with NC were: 1.08x10⁶±2x10⁵; 2.59x10⁶±1x10⁶ and 2.6x10⁶±1x10⁶ PFU/ml. (D) Infectious WNV production at 12 hpi; in cells transfected with the NC the viral titers were 1.82x10⁵±1x10⁴ and 4.14x10⁵±1.32x10⁵ PFU/ml in upon infection at MOIs 0.5 and 1, respectively. Data is presented as the percentage relative to the NC and shows mean ± SEM from at least three independent experiments. Statistical differences were assessed with Student’s t-test.