Fig 6. Overexpression of miR-3614-5p downregulates ADAR1 expression in Huh7 cells.

(A) Huh7 cells were transfected with the mimic of miR-3614-5p or a siRNA against ADAR1 (siADAR). The correspondent negative control (NC) mimic and NC siRNA were also used. At 24 hpt, total protein was extracted and ADAR1 expression was detected by western blot. (B) Huh7 cells were transfected with the mimic of miR-3614-5p and NC. At 24 hpt, cells were infected with DENV at MOI 1 and 10. At 24 hpi, total protein was extracted and ADAR1 expression was detected by western blot. (A, B) The expression of ADAR1 was normalized to that of GAPDH and it is expressed as the percentage of the cells transfected with the correspondent NC. Data shows mean ± SEM from three independent experiments. Differences were assessed with Student’s t-test.