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. 2017 Nov 1;28(22):2978–2997. doi: 10.1091/mbc.E17-02-0101

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© 2017 Escorcia and Forsburg. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Nondisjunction in MI and MII in FPC mutants. (A) Live-cell images of meiotic cells carrying fluorescently tagged histone3 (Hht1-mRFP) and LacI-GFP on a LacO repeat at lys1 near the centromere of chromosome I. Panels are divided into individual fluorescent channels. Bright-field panels are omitted due to space limitations. A false-color image is provided to show merged signals. Dotted cell outlines are overlaid on panels for easier visualization of meiotic cells. Images that show the characteristics of each reported mutant phenotype were used. Displayed time frames were chosen for optimal representation of nuclear dynamics. The meiotic phases shown are HT, metaphase (MT), MI, and MII. Minute 0 (0′) denotes the last nuclear mass contraction in metaphase I before homologous chromosomes separate in anaphase I (10′). Upright scale bars: 5 µm. (B) Cartoon depicting a pair of homologous chromosomes marked with GFP near the centromeres. Asymmetric distribution of GFP indicates nondisjunction events. (C, D) Quantification of cells showing chromosome nondisjunction in MI and MII. More than 150 cells were scored from at least two independent movies for each genotype. Chi-squared analysis was used to determine significance. p values are reported as follows: *, p < 0.05. Error bars represent 95% confidence intervals.