FIGURE 7:

Duration of Sgo1 in metaphase in FPC mutants. (A) Live-cell images of meiotic cells carrying fluorescently tagged histone3 (Hht1-mRFP) and Sgo1-GFP. Panels are divided into individual fluorescent channels. Bright-field panels are omitted due to space limitations. A false-color image was generated to show merged signals. Dotted cell outlines are overlaid on panels for easier visualization of meiotic cells. Images that show the characteristics of each reported mutant phenotype were used. Displayed time frames were chosen for optimal representation of nuclear dynamics. The meiotic phases shown are HT, metaphase (MT), and MI. Time preceding the last nuclear mass contraction in metaphase I (0′) before homologous chromosomes separate in anaphase I (10′) is denoted with a negative sign (e.g., −10′). Upright scale bars: 5 µm. White arrows indicate average span of Sgo1-GFP signal. (B) Cartoon depicting duration of Sgo1-GFP signal during metaphase and before MI. (C) Quantification of Sgo1-GFP fluorescence. (D) Quantification of Sgo1-GFP foci in metaphase I. (E) Duration of Sgo1-GFP foci. More than 150 cells were scored from at least two independent movies for each genotype. One-way ANOVA followed by Tukey family-wise comparison was used to determine significance in C and E. Chi-squared analysis was used for D. p values are reported as follows: *, p < 0.05. Error bars represent 95% confidence intervals.