TABLE 1:
Recombination dynamics in FPC mutants with intergenic and intragenic recombination data given in the top panels and sister chromatid recombination data given in the bottom panel.
| Genetic distance (cM)a | Fold reductionb | p valuec | ||||||
|---|---|---|---|---|---|---|---|---|
| Intergenic interval | Chr | WT | swi1∆ | swi3∆ | swi1∆ | swi3∆ | swi1∆ | swi3∆ |
| his4-lys4d | 2 | 6.81 ± 0.92 | 4.81 ± 0.78 | 2.43 ± 0.83 | 1.42 | 2.80 | 0.0039 | p << 1E-04 |
| leu2-ura2d | 1 | 0.87 ± 0.13 | 0.67 ± 0.20 | 0.40 ± 0.24 | 1.30 | 2.18 | 0.1164 | 0.0051 |
| leu1-his7d | 2 | 2.28 ± 0.41 | 1.08 ± 0.34 | 1.21 ± 0.20 | 2.11 | 1.88 | 0.0004 | 0.0005 |
| his3-leu1e | 2 | 1.13 ± 0.22 | 0.55 ± 0.08 | 0.92 ± 0.08 | 2.05 | 1.23 | 0.0008 | 0.1078 |
| Intragenic intervalf | Chr | Frequency (×10−3) | Fold reductionb | p valueg | ||||
|---|---|---|---|---|---|---|---|---|
| ade6-M26-52 | 3 | 3.34 ± 0.08 (60/17987) | 1.70 ± 0.05 (52/31129) | 0.60 ± 0.04 (7/11838) | 1.96 | 5.57 | 0.0009 | P << 1E-04 |
| Frequency (×10−3) | Fold increaseh | p valueg | ||||||
|---|---|---|---|---|---|---|---|---|
| SCE intervalf | Chr | WT | swi1∆ | swi3∆ | swi1∆ | swi3∆ | swi1∆ | swi3∆ |
| ade6-L469-his3+-ade6-M375/ade6-M375-M210 | 3 | 5.00 ± 0.08 (167/33465) | 16.81 ± 0.24 (186/11064) | 9.13 ± 0.14 (155/16982) | 3.36 | 1.83 | P << 1E-04 | p << 1E-04 |
Significance for genetic distance was determined using a two-tailed Student’s t test. Chi-squared analysis was employed for frequencies. At least five independent trials were carried out per genotype. Error margins represent 95% confidence intervals. Fold change values are relative to wild-type outcomes. At least five independent trials were performed per genotype. Chr, chromosome; WT, wild type.
aHaldane’s formula cM = −50 ln(1−2R) was used to convert recombinant frequencies (R) into centimorgans (cM).
bWild-type cM or frequency values were divided by those of mutants to determine reduction in genetic distance or recombination frequency.
cSignificance calculated by two-tailed student’s t test.
dMarkers in interval were scored in repulsion (+−/−+).
eMarkers in interval were scored in coupling (++/– –).
fFrequency values were calculated by dividing number of Ade+ by total number of colonies screened (shown in parentheses).
gSignificance calculated by chi-squared analysis.
hFrequency values of mutants were divided by those of the wild type to determine increase in recombination frequency.