FIGURE 5:

Bafilomycin A1, a V-ATPase inhibitor, blocks vacuolar acidification downstream of their formation. (A) Representative DIC images of vacuole formation induced by shear overnight, comparing BafA with vehicle-treated cell monolayers. (B) Quantification of the number of vacuoles formed resulting from shear induction overnight or static control, comparing monolayers treated with vehicle and BafA. Error bars represent SEM from n = 3 independent experiments. Data scales are normalized values derived from mean centering and variance scaling of each set of experiment. (C) Distribution of vacuole diameter measured by image analysis of experiments in A. Data represent combination of all vacuoles from n = 3 experiments. **P < 0.05 by t test.