FIGURE 8:

Perturbing microvilli by CDHR2 knockdown suppressed vacuole formation and autophagic trafficking. (A) Representative scanning electron micrographs of the apical surfaces of cell monolayers cultured overnight, comparing CDHR2 knockdown with scramble control. (B) Representative DIC images of shear-induced vacuole formation overnight, comparing CDHR2 knockdown with scramble control. (C) Quantification of vacuole number as a time course of shear stress or static control, comparing conditions outlined in B. Statistical analysis was performed by two-way ANOVA. (D) Quantification of the number of dextran-positive vacuoles resulting from overnight shear induction or static control, comparing conditions outlined in B. (E) Representative IF images (as a maximum Z-projection) of LC3 (green) expression induced by shear overnight, comparing CDHR2 knockdown with scramble control. (F) Quantification of LC3+ particles in E. Error bars represent SEM from n = 3 independent experiments. Data scales are normalized values derived from mean centering and variance scaling of each set of experiments. **P < 0.01, ****P < 0.0001 by t test.