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Model for the mode of action of Zonda in omegasome formation. From left to right: Shortly after starvation Zda nucleates on the ER and the Zda-labeled sites function as platforms for Vps34-dependent PI3P deposition. ER domains enriched in PI3P evolve into ring-shaped omegasomes from which phagophores containing Atg5 and Atg8 bud off.
