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. 2017 Nov 1;28(22):3070–3081. doi: 10.1091/mbc.E16-11-0767

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© 2017 Melani, Valko, Romero, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Starvation-induced DFCP1 deposition requires Zonda. Confocal images of salivary gland cells of fed (A) or starved (B–D) third instar larvae expressing GFP-DFCP1 (green) and labeled with rhodamine-phalloidin (red). white^RNAi (A and B), zda^RNAi (C), or a vps34^KD (D) were coexpressed. Starvation induced an increase in the number of DFCP1 foci in white^RNAi larvae, indicating omegasome formation (A and B). Zda silencing (C) or Vps34 activity blockage (D) abolished this increase. The number of GFP-DFCP1 foci per cell was quantified in each condition (E), N = 40. Data represent mean ± SD. Means marked with an asterisk are significantly different. One-way ANOVA followed by a Tukey´s test with a confidence interval higher than 95% (p < 0.05). Scale bar: 20 μm.