FIGURE 8:

CAV1 incorporates correctly into CAV1/CAV2 hetero-oligomers in patient fibroblasts as assessed by BN–PAGE. Control and patient skin fibroblasts were subjected to BN–PAGE and blotted for the indicated proteins. Untransfected HeLa cells and HeLa cells transiently expressing EGFP, CAV1-GFP, or P132L-GFP were used as controls. Equal amounts of protein were loaded in each lane. (A) Western blots from BN–PAGE were blotted using CAV1 N-term (red in merge) or an anti-GFP antibody (green in merge). The red arrow indicates the position of complexes containing endogenous CAV1, and the green arrow shows complexes containing CAV1-GFP or P132L-GFP. Data are representative of at least three independent experiments. (B) As in A except blots were probed using CAV1 N-term (red in merge) and an antibody against CAV2 (green in merge). Note the strong overlap between CAV1 and CAV2 signals in the merged image. Data are representative of at least two independent experiments.