Table 2.
Apparent kinetics parameters for SpNanC and BiNanH2.
| Sialidases | Substrate | kcat (s− 1) | KM (mM) | kcat/KM (s− 1 mM− 1 ) |
|---|---|---|---|---|
| SpNanC | Neu5Acα2–3GalβpNP (17) | (3.65 ± 0.10)×102 | 2.66 ± 0.3 | 137 |
| Neu5Ac9NAcα2–3GalβpNP (1) | (3.38 ± 0.15)×102 | 2.60 ± 0.4 | 130 | |
| Neu5,9Ac2α β2–3Gal pNP (19) | (3.78 ± 0.12)×102 | 1.25 ± 0.2 | 302 | |
| BiNanH2 | Neu5Acα2–3GalβpNP (17) | (1.66 ± 0.02)×102 | 2.8 ± 0.1 | 59.3 |
| Neu5Ac9NAcα2–3GalβpNP (1) | (1.08 ± 0.02)×102 | 1.61 ± 0.2 | 66.7 | |
| Neu5Acα2–6GalβpNP (18) | (3.31 ± 0.06)×102 | 1.37 ± 0.1 | 242 | |
| Neu5Ac9NAcα2–6GalβpNP (8) | (8.07 ± 0.08)×102 | 4.51 ± 0.2 | 179 |