Table 1. Apparent kinetic parameters of Hp3/4FT.
Substrate | kcat (s−1) | KM (mM) | kcat/KM (s−1 mM−1) |
---|---|---|---|
LacβPro2AA | 0.54±0.03 | 0.99±0.18 | 0.55 |
aGDP-Fuc | 0.30±0.14 | 0.38±0.07 | 0.80 |
LacNAcβPro2AA | 5.00±0.18 | 0.46±0.07 | 10.87 |
bGDP-Fuc | 6.02±0.26 | 0.86±0.13 | 7.01 |
Galβ3GlcNAcβPro2AA | 7.77±0.45 | 1.32±0.24 | 5.89 |
cGDP-Fuc | 3.92±0.16 | 0.35±0.06 | 11.19 |
Determined using LacβPro2AA as an acceptor
Determined using LacNAcβPro2AA as an acceptor
Determined using Galβ3GlcNAcβPro2AA as an acceptor.