Figure 13.

Reversible photopatterning of full length proteins using sequential photoconjugation and photocleavage reactions. a) Network alkoxyamines are uncaged and react with proteins conjugated to photocleavable aldehydes. A secondary round of light exposure releases the protein. b) A second protein can be incorporated during the release of the first, forming intricate patterns. Scale bar 50 μm. c) Sustained vitronectin immobilization leads to osteogenic differentiation of hMSCs, indicated by staining for osteocalcin (green). Reprinted from DeForest et al.⁹⁶ copyright (2015) Nature Publishing Group.