Figure 6.
Reversible thiol-ene photopatterning via allyl sulfide chemistry. a) Consecutive swapping of thiolated molecules can be performed, forming new thioether bonds and reactive alkenes. b) An RGD peptide containing a cysteine residue and AlexaFluor555 is tethered to the network by two-photon photolithography. c) A second thiolated peptide (with AlexaFluor488 dye) is photopatterned in the buffalo shape, simultaneously releasing the first peptide. d) A third peptide (unlabeled) is patterned in the shape of the CU logo, releasing the second peptide. Scale bar 100 μm. Adapted from Gandavarapu et al.176 copyright (2014) Wiley.