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. 2017 Oct 26;8:1371. doi: 10.3389/fimmu.2017.01371

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Copyright © 2017 Wiesinger, Stoica, Roessner, Lorenz, Fischer, Atreya, Neufert, Atreya, Scheffold, Schuler-Thurner, Neurath, Schuler and Voskens.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Assessment of regulatory T cell (Treg) drug product purity. (A) Representative FACS plots gated on PE⁺/APC⁺ anti-CD3/anti-CD28 expander beads in a control sample containing no anti-CD3/anti-CD28 expander beads (=background sample), a control sample containing 750 anti-CD3/anti-CD28 expander beads (=negative control sample), a control sample containing 1,000 anti-CD3/anti-CD28 expander beads (=standard sample), and a control sampler containing 3,000 anti-CD3/anti-CD28 expander beads. (B) FACS plots gated on PE⁺/APC⁺ anti-CD3/anti-CD28 expander beads in a thawed Treg drug product from consistency run 1 (=Con1), consistency run 2 (=Con2), consistency run 3 (=Con3), and consistency run 4 (=Con4).