Figure 6.

Monocytes contribute a significant proportion of transcriptional changes detected in peripheral blood mononuclear cells (PBMC). (A) Principal component analysis of normalized transcript numbers 6 DPI in PBMC, monocytes, T-cells, and B-cells. (B) 4 way Venn diagram showing overlap between differentially expressed genes (DEGs) detected 6 DPI in PBMC, monocyte, T-cells, and B-cells. (C) Heatmap representing gene expression (shown as absolute normalized RPKM values) of the 24 DEGs detected in PBMC as well as purified monocytes, T- and B-cells 6 DPI; range of colors is based on scaled and centered RPKM values of the entire set of genes (red represents increased expression while blue represents decreased expression); each column represents the median RPKM values on each day for each subset. (D) Violin plot of the log₂FC of 971 DEGs that were exclusively detected in PBMC within each cell subset.