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. 2017 Oct 30;7:14371. doi: 10.1038/s41598-017-14652-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of HBeAg expression on cell cycle profile in Huh7 cells using flow cytometry. Huh7 cells transfected with the (A) control (no HBeAg) or (B) pCMVHBeAg were analysed by PI staining and flow cytometry (The M1 peak corresponds to cells in G0/G1, M3 peak corresponds to cells in G2/M and cells in S phase are shown as M2). (C) A bar graph showing the proportion of cells in different phases of cell cycle in the presence (pCMVHBeAg) and absence (control) of HBeAg as analysed by flow cytometry (the bar graphs are represented as mean ± SD with n = 3; NS = not significant).