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. 2017 Oct 30;7:14371. doi: 10.1038/s41598-017-14652-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of microarray findings on miR-106b and the Rb gene. (A) Quantitation of miR-106b expression levels in cells transfected with pCMVHBeAg (expresses HBeAg) or the control (no HBeAg) using real time PCR. (B) Real time PCR quantitation of Rb mRNA levels (normalized using GAPDH levels) in cells expressing HBeAg (pCMVHBeAg) and in control cells (transfected with the control plasmid). (C) Western Blot analysis of pRb expression in Huh7 cells (The full-length western blots images are shown in supplementary Fig. S2; the bar graphs are represented as mean ± SD with n = 3).