Fig. 3.

Overexpression of TGFBR3 and PLAG1 causes transformation of human salivary gland (HSG) cells. a RT-QPCR of TGFBR3 and PLAG1 mRNA levels in HSGs infected with constructs expressing combinations of PLAG1 or the corresponding empty vector (EV) 1, and TGFBR3 or the corresponding EV2. Data are mean using three technical replicates, normalized to the reference gene STLM. b Proliferation. Data are mean ± SEM using three technical replicates. *P < 0.05, **P < 0.01, ***P < 0.001; two-way ANNOVA with Dunnett’s multiple comparison test. The experiment was replicated three times. c Migration index. Data are mean ± SEM using four technical replicates. *P < 0.05; two-way ANNOVA with Dunnett’s multiple comparison test. The experiment was replicated two times. d Representative photographs (left) and quantification (right) of soft agar colony formation. Data are mean ± SEM from three technical replicates. *P < 0.05; one-way ANNOVA with Tukey’s multiple comparison test. The experiment was replicated two times