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. 2017 Oct 30;7:14360. doi: 10.1038/s41598-017-14307-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NET formation in thrombi 48 hours after IVC ligation was reduced in pcsk9 ^−/− mice compared to WT mice (n = 5 mice per group). (A,B) Representative photomicrograph of cathelicidin-related antimicrobial peptide (CRAMP) staining from WT (A) and pcsk9 ^−/− (B) mice. (C) Quantification of CRAMP-positive cells per unit area. (D,E) Representative photomicrograph of citrullinated histone H3 (Cit-H3) staining from WT (D) and pcsk9 ^−/− (E) mice. (F) Quantification of Cit-H3-positive cells per unit area. *P < 0.05. **P < 0.01. Scale: 50 μm.