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. 2017 Oct 30;7:14336. doi: 10.1038/s41598-017-14298-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Macrophage accumulation in RD is not affected by ISO-1. (a) Immunoperoxidase photomicrographs of F4/80+ cells (red) in the retina of C57BL/6 mice systemically treated with ISO-1 or vehicle demonstrates an accumulation of inflammation in detached retina compared with attached fellow-eye controls at day 3 and 14 (n = 6/group). (b) Quantification reveals the F4/80+ cell count was not affected by ISO-1 treatment compared with vehicle treatment at either timepoints (p = 0.2293, day3 and p = 0.4714, day14). Scale bar denotes 50 microns.