Table 2.
Oligonucleotides used in this work.
| Name | Sequence (5′-3′)a | Nucleotide positonsb |
|---|---|---|
| HexAF22 | GGCATGCAACAGTATGTGG | 64 to 82 of hexA |
| HexAF757 | CCAAGATTGCTGGCTTGCCAGC | 2,330 to 2,352 of hexA |
| HexAR755 | CCAGCAATCTTGGCAACATGG | Complementary to 2,342 to 2,321 of hexA |
| HexARTF | GCTCTTTCGGATGGGTGATTT | 113 to 134 of hexA |
| HexARTR | GCTTACTGCTATCGACCACTGT | Complementary to 378 to 400 of hexA |
| HexARTF2 | CATCCTATCCTAGCAGAATTAGC | 2,479 to 2,501 of hexA |
| HexARDown | TTATAGTTTCTGTTTTAACTCTAC | Complementary to 2,551 to 2,574 of hexA |
| GuaR80 | GGATGAACATTTTGGCCTCCTG | Complementary to 474 to 452 of guaA |
| PatAF17 | GATGACAGGCTTGATGGTTGC | 53 to 74 of patA |
| PatAR17 | GCAACCATCAAGCCTGTCATC | 74 to 53 of patA |
| PatBFUp | CGCATGCAGACTTGGTTGCCA | −827 to −806 of patB |
| PatARDown | TGGCAACCAAGTCTGCATGCG | Complementary to −806 to −827 of patB |
| PatBRDown | TAGGACAAGAAAAAGCAGCCCC | Complementary to −73 to −51 of guaA |
| PatARTF | TGATGACAGGCTTGATGGTT | 52 to 72 of patA |
| PatARTR | AACGACTAGATTTCCCGCAT | Complementary to 333 to 313 of patA |
| PatARTR2 | AACCATCAAGCCTGTCATCA | Complementary to 53 to 72 of patA |
| PatARACE1 | CTCCTTGGGCAATATAGGCTGC | Complementary to 220 to 241 of patA |
| PatARACE2 | CCAATCAACCAAGCCCCGATAC | Complementary to 152 to 173 of patA |
| PatBRTF | GCACCCCATTGGCTTTCCTTA | 523 to 542 of patB |
| PatBRTR | GCACGCGCTCATTTTGTTCA | Complementary to 715 to 695 of patB |
| rDNAF | GGTGAGTAACGCGTAGGTAA | 101 a 120 of 16SrDNA |
| rDNAR | ACGATCCGAAAACCTTCTTC | Complementary to 407 to 426 of 16SrDNA |
| RibAF135 | GCTTCTGTGTGTCCATTTCTTTC | 411 to 434 of ribA |
| RACE 5′ | GATATGCGCGAATTCCTGTAG | 1 to 21 of RNA adaptor |
| RACE5′Inner | AATTCCTGTAGAACGAACACTAGAA | 11 to 35 of RNA adaptor |
| RNA adaptor | GAUAUGCGCGAAUUCCUGUAGAACGAACACUrArGrArArArGrArArA | |
| TagAR131 | GGGGCTTGGCGATAATCAGG | Complementary to 413 to 393 of tagA |
a
The “r” before the base indicated that it was a ribonucleotide.
b
Nucleotide and amino acid numbering refers to the genes and proteins obtained from the S. pneumoniae R6 sequence, with the first nucleotide or amino acid at position 1.