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. 2017 Oct 30;8:1196. doi: 10.1038/s41467-017-01371-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Compatibility of the setup with two-photon imaging. a Left panel: side view schematic of the latching unit for physiology with a ×10 objective lens for two-photon imaging. The two vertical gray segments indicate the latching pins (Supplementary Fig. 6). Right panel: 3D rendering of the latching part of the unit. b Wide-field GCaMP8 fluorescence image at the cortical surface. Red rectangle, ROI within V1. Scale bar 1 mm, dotted yellow lines and labels are segmented visual areas (Methods). c Repeated imaging of the same ROI across different days. Blue squares are magnified views of two example cells. Scale bar, 200 μm. d Visually evoked responses during the behavioral task recorded from cell1 shown in c. ΔF/F trial averages for left/right choices (red/blue lines). Shaded areas, 95% confidence interval. Time-out trials were not included in the data. e Responses to oriented gratings (30° diameter) from cell-2 shown in c. Error bars are s.e.m. Red curves, Gaussian fit to the data. Original technical drawings in a edited with permission from O’ Hara & Co., Ltd.