Figure 7.

mDC^hCPC and mDC^IFNγ-hCPC produce IL10 rather than TNFα and immune-modulate an ongoing immune response. (A) The levels of IL10 and TNFα were quantified by specific enzyme-linked immunosorbent assay from supernatants of co-cultures conducted as in Figure 6. (B) Modulation of PHA-induced T cells proliferation by mature DC (mDC) in the absence (medium) or the presence of hCPC (hCPC) or IFNγ-hCPC (IFNγ-hCPC) was determined by immunomodulation assays described under Section “Materials and Methods.” T cells proliferation was analyzed by flow cytometry as in Figure 4. Results are presented as division index compared to PHA or medium (lower panel) and representative histograms in the upper panel. Data on the graphs represent the mean ± SEM from four independent experiments conducted with Peripheral blood mononuclear cells (PBMC) isolated from four different donors and are representative of data obtained with two other hCPC from different donors. Each data point represents the mean of experimental triplicates. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns: not significant.