Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Oct 30;7:14296. doi: 10.1038/s41598-017-14512-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Purification and crystallization of Nb9-bound BtuF. (A) Size exclusion chromatography of the Nb9-BtuF complex. Note the shift of the elution peak of the Nb9-BtuF complex compared to the individual proteins. Equivalent amounts of protein (710 μg BtuF, 3.2 mg Nb9) were injected in all experiments. (B) SDS-PAGE analysis of isolated Nb9-BtuF complex after gel filtration confirmed the presence of both components. (C) Crystals of the Nb9-BtuF complex grown in optimized screens containing 100 mM Tris-HCl pH 8.5, 400 mM MgCl₂ and 33% w/v PEG4000.