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. 2017 Oct 30;7:14296. doi: 10.1038/s41598-017-14512-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of Nb9-BtuF complex with Cbl-bound BtuF. Nanobody-bound BtuF is shown in light blue cartoon representation, with the nanobody in green with colored CDRs (CDR1, yellow; CDR2, magenta; CDR3, salmon). Cbl-bound BtuF (PDB ID 1N4A) is displayed in red and Cbl is shown in stick representation with oxygen in red, nitrogen in blue, and cobalt in brown. The two BtuF chains superimposed with an RMSD of 1.53 Å over 243 atoms. Distances of Cα position between corresponding atoms in the loops gating the entrance of the substrate-binding pocket are indicated (1: 5.5 Å, Q67 in Nb9-BtuF and 2: 4.4 Å, N165 in Nb9-BtuF). The C-lobe of BtuF displays more pronounced structural changes than the N-lobe.