Table 4.

Identification of differentially regulated proteins in the control (ddH₂O) and treatment (1600 mg/L AgNPs) groups

Spot no.a	Protein ID	Name	Gene name	Theoretical MWb (kDa)/pIc		ANOVA P value	Fold changed	Expresse
1	gi|512908327	Calexcitin-2-like	/	23.56	5.09	2.09E−04	2.03	↓
2	gi|13195043	Fibroin light chain, partial	Fib-l	24.83	4.53	0.00108697	2.28	↓
3	gi|512931543	Ubiquitin carboxyl-terminal hydrolase	/	25.1	5.02	2.82E−04	2.01	↓
4	gi|512915932	Cytosolic non-specific dipeptidase	/	58.83	6.15	6.94E−05	2.27	↓
5	gi|112983926	Arginine kinase	AK	32.50	7.23	1.36E−05	0.43	↑
6	gi|512915980	S-formylglutathione hydrolase	/	32.13	5.65	0.00128389	5.25	↓
7	gi|827538302	Low molecular 30 kDa lipoproteinPBMHP-12	Lp-c12	21.83	8.61	0.0338263	6.38	↓
8	gi|225905552	Low molecular lipoprotein 30K pBmHPC-6	Lp-c6	29.82	5.92	0.0464862	2.73	↓
9	gi|87248167	Isocitrate dehydrogenase, partial	/	46.55	6.24	0.00947802	11.25	↓
10	gi|112983420	Heat shock protein hsp 19.9	Hsp19.9	19.94	6.53	2.40E−04	2.67	↓
11	gi|827541166	Arginine kinase	AK	40.31	5.87	3.57E−04	0.27	↑
12	gi|112983028	Glutathione S-transferase sigma 1	GSTs1	23.60	5.98	0.00239861	0.48	↑
13	gi|6016405	Juvenile hormone-binding protein	JHBP	2.15	6.02	2.89E−05	0.44	↑

^aNumbers indicate regions that were excised from the SDS-polyacrylamide gels for the mass spectrometry analysis

^bMolecular weight

^cIsoelectric point

^dFold change = control/treatment

^eUpregulated expression “↑”; downregulated expression“↓”