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. 2017 Oct 30;7:14334. doi: 10.1038/s41598-017-14699-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Distribution of EYFP-labelled interneurons and specific co-localization with SOM. (a) Gene elements of the Ai3 Cre-reporter mouse and SOM-Cre-line mouse used in the experiments. (b) Representative examples of specific co-localization of EYFP-labelled neurons (top, green) from a SOM-IRES-Cre:Ai3-EYFP mouse with immunofluorescence for somatostatin (middle, red). Merged images are shown at the bottom. Scale bars, 100 µm. Nearly all of the EYFP-labelled neurons from SOM-IRES-Cre:Ai3-EYFP mice co-localized with somatostatin in MOp, HPF and CP. Abbreviations: MOp, Primary motor area; HPF, Hippocampal formation and CP, Caudoputamen. SOM-positive rates in MOp, HPF and CP were 74.27 ± 1.31% (n = 3), 80.80 ± 1.39% (n = 3) and 77.53 ± 2.19% (n = 3), respectively.