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. 2017 Oct 30;7:14334. doi: 10.1038/s41598-017-14699-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Whole-brain imaging of a SOM-IRES-Cre:Ai3-EYFP mouse brain. Images of EYFP-labelled neurons (a) and PI-stained cytoarchitecture (b) in the hippocampal coronal plane. (c) Enlarged views of white rectangular boxes indicated in (a) and (b) and their merged image. Image size is 900 × 400 μm. Projection thickness in the EYFP channel was 20 μm. (d) Sagittal reconstruction of maximum intensity projections of the SOM-IRES-Cre:Ai3-EYFP mouse brain. The projection thickness shown in (d) is 20 μm. The insets (d1)–(d5) are enlarged views of white square boxes shown in (d). The sizes of (d1)–(d5) are 400 × 400 × 20 μm. Scale bar: (a) and (b) 1 mm, (c) 25 μm, (c1)–(c3) 50 μm, (d) 1 mm and (d1)–(d5) 100 μm.