Table 2.
Time and estimated cost analysis for rDNA sequencing from one isolate using conventional Sanger sequencing versus MALDI-TOF-MS biotyping.
| DNA sequencing (Sanger) | MALDI-TOF-MS | ||
|---|---|---|---|
| Hands-on time | Hands-off time | Hands-on time | Hands-off time |
| Process/work time | |||
| DNA extraction | Protein extraction | ||
| 20 min | 0 min | ||
| Nested PCR steps | 1 min | 5 min | |
| 40 min | 5 hr | ||
| Cloning steps | MALDI sampling | ||
| 3–4 hr | 1–2 days | ||
| Sequencing reaction | 10 min | 5 min | |
| 25 min | 4 hr | ||
| Sequencing clean up | Data acquisition | ||
| 30 min | 0 hr | ||
| Sanger Sequencing | 5 min | 1 min | |
| 5 min | 1 hr | ||
| Total time | |||
| Total time/sample | Total time/sample | ||
| 5–6 hr | 34–58 hr | 16 min | 11 min |
| Approximate cost | |||
| (Chemistry and consumables) | |||
| Total Price/sample | Total Price/sample | ||
| 17 US $ with cloning | 3 US $ | ||
| 9.5 US $ without | |||
| Approximate cost | |||
| (Instruments) | |||
| 95000 US $ | 155000 US $ | ||