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. 2017 Oct 30;8:1189. doi: 10.1038/s41467-017-01126-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Sfpq mediates the interaction between miRISC and Pspc1 or NonO. a Co-IP of endogenous Ago2 with Sfpq, Pspc1, or NonO from P19 cells. Cells were transfected with the indicated siRNAs and analyzed by Western blotting. Ago2 and tubulin served as controls. b RNA-IP of let-7a with the endogenous Sfpq, Pspc1, or NonO. RAW 264.7 cells were transfected with the indicated siRNAs. Cell extracts were immunoprecipitated with the indicated antibodies and the RNA was purified and analyzed by Northern blotting