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. 2017 Oct 30;7:14389. doi: 10.1038/s41598-017-14691-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Experiment design (a) and example output (b; DatLab software by Oroboros, Innsbruck, Austria) from a high-resolution respirometry experiment on the Oxygraph-2k. The y-axis shows O₂ flux per mass (pmol ▪ s⁻¹ ▪ mg⁻¹), with time on the x-axis. The SUIT protocol for this study was as follows: glutamate + malate (10 mM and 2 mM; LEAK, state 2), ADP (2.5 mM; P_CI), succinate (10 mM; P_CI+II), cytochrome c (10 μM), ADP + succinate (2.5 mM and 10 mM), 0.5 μM titrations of FCCP (E_CI+II), and antimycin A (2.5 μM; ROX). O₂ concentration was maintained between 200 and 500 pmol/mL throughout the experiment.