Fig. 1.

Drug responses based on transcriptional signatures can be cell type specific or universal. A network of consistent L1000 signatures (SCS > 1.3) scored using the characteristic direction approach. Each node represents a unique perturbation (a combination of drug, cell line, time point and concentration) and edges are drawn between perturbations having a cosine distance in the lower 2-percentile. Color is based on cluster identity, size denotes concentration, and shape denotes the time point. For each cluster, a box labeled with the cluster number illustrates the distribution of the perturbation by cell line (left bar) and drug target class (right bar). Target classes are assigned based on the nominal targets of a drug and do not consider potential poly-pharmacology. The drugs assigned to each class are listed in Supplementary Data 1. An interactive version of this figure is available at http://amp.pharm.mssm.edu/LJP