Figure 6.

Cellular infiltration in lungs after lethal virus infection. The immunized mice (n=5) were infected with A/PR8 virus (2×LD50) after 6 weeks of immunization. Lung samples were harvested day 7 post infection, and cell phenotypes were determined by flow cytometry and calculated by multiplying cell percentages with total cell numbers. (A) Total lung cells. (B) AMs; CD11b⁻CD11c⁺F4/80⁺. (C) Monocytes; CD11b⁺F4/80⁺Ly6C^high. (D) Neutrophils; CD11b⁺F4/80⁻Ly6c⁺. (E) pDCs; CD45⁺F4/80⁻CD11c⁺MHCII^highB220⁺. (F) CD103 ⁺ DC; CD45⁺F4/80⁻CD11c⁺MHCII^highCD11b⁻CD103⁺. (G) cDC; CD45⁺F4/80⁻CD11c⁺MHCII^highCD11b⁺. All results were shown in mean±SEM. For statistical analysis, One-way ANOVA and Tukey's post-multiple comparison tests were performed.

pDC, plasmacytoid dendritic cell; cDC, conventional DC.

^*p<0.033; ^**p<0.002 compared to naïve infection group; ^†p<0.033 compared to sPR8 group.