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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Eur J Immunol. 2017 Apr 13;47(5):800–805. doi: 10.1002/eji.201646871

Figure 2. Thymic DX5+ ILC-like cells are RAG1-independent.

Figure 2

(A) FACS analysis for ILC-like cells in Rag1−/− mice. (B) Thymocytes were enriched for CD8− cells and analyzed for expression of NK1.1 and the NKT-cell receptor using PBS57 (left) or unloaded (right) CD1d tetramers. (C) CD49a and CD103 on NK1.1+ and CD1dPBS57+ (NKT1) or CD1dPBS57− (Tet−NK1.1+) cells. (D) Expression of CD127, CD11b, CD49a, CD103, EOMES and GATA3 is shown for DX5+ tNK cells. Open profile is the FMO. (E) Mean percent ± SEM of thymic ILC-like cells expressing the indicated proteins in Rag1+/+ (+, black) or Rag1−/− (-, grey) mice. Each dot represents one mouse. (A, D) Data are representative of 4–7 experiments, (B, C) representative of 3 experiments with one mouse of each genotype. Unpaired t-test * p<0.05, **p<0.01.