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. 2017 Oct 25;8:287. doi: 10.3389/fendo.2017.00287

Figure 4.

Figure 4

Neonatal overfeeding effects on neonatal hypothalamic responses to leptin. (A) Arcuate nucleus of the hypothalamus (ARC) and (B) ventromedial hypothalamus (VMH) neuronal activation in response to leptin injection at postnatal day (P) 12 in control (CL) and small litter (SL) rats as assessed by numbers of signal transducer and activator of transcription (pSTAT3) positive cells. The sum of cell counts in four sections was plotted. (C) Schematic diagram adapted from Paxinos and Watson illustrating the regions of interest. Thick-line boxes are representative of analyzed regions, dotted-line boxes are representative of the photomicrographs. Representative photomicrographs of pSTAT3 after a saline or leptin injection in the (D) ARC (scale bars = 100 µm) and (E) VMH (scale bars = 200 µm). Data are mean ± SEM. n = 5–6 per group. # significant main effect of litter size. % significant main effect of leptin. p < 0.05.